This amido black water-based formula is a one-step process that includes a blood fixer. The sensitivity and color intensity of the process are similar to that of the amido black methanol-based formula. This process uses a tap water rinse.
Equipment
Scales, beakers, graduated cylinder, magnetic stirrer and stirring bar, squirt bottle or sprayer, clear or dark storage bottles
Materials and Chemicals
Glacial acetic acid
Formic acid (concentrated)
Naphthol blue black
Kodak Photo-Flo 600 solution
Sodium carbonate
5-Sulfrosalicylic acid (purity equal to or greater than 99%)
Mixing Procedures
Amido black water-based one-step formula is prepared on a stirring device by combining the ingredients in the order that they are listed.
Distilled water - 500 mL
5-Sulfosalicylic acid - 20 g
Naphthol blue black - 3 g
Sodium carbonate - 3 g
Formic acid - 50 mL
Glacial acetic acid - 50 mL
Kodak Photo-Flo™ 600 solution - 12.5 mL
Dilute this mixture to 1 L using distilled water. Although this mixture will be ready to use following dilution, allow the mixture to stand for several days prior to use for best results.
Processing Procedure
Apply the amido black to the specimen(s) by dipping or using a squirt bottle. Leave the amido black on the specimen(s) for 3 to 5 minutes, then rinse using tap water. These steps can be repeated for the desired contrast.
Storage
Clear of dark bottles
Shelf Life
Indefinite
Disposal
Observe all federal, state, and local environmental disposal regulations. State and local disposal regulations may differ from federal disposal regulations.